Experimental Methods in Systems Biology (Coursera)

Experimental Methods in Systems Biology (Coursera)
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Experimental Methods in Systems Biology (Coursera)
Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging. A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses.

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We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content.

Course 2 of 6 in the Systems Biology and Biotechnology Specialisation.


Syllabus


WEEK 1: Introduction

WEEK 2: Deep mRNA Sequencing

WEEK 3: Mass Spectrometry-Based Proteomics

WEEK 4: Midterm Exam

WEEK 5: Flow and Mass Cytometry for Single Cell Protein Levels and Cell Fate

WEEK 6: Live-cell Imaging for Single Cell Protein Dynamics

WEEK 7: Integrating and Interpreting Datasets with Network Models and Dynamical Models

WEEK 8: Final Exam



MOOC List is learner-supported. When you buy through links on our site, we may earn an affiliate commission.

Course Auditing
32.00 EUR/month

MOOC List is learner-supported. When you buy through links on our site, we may earn an affiliate commission.